Genetics of Cell‐Mediated Lympholysis (CML) in Man

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Abstract
Human cytotoxic lymphocytes (CTL), developed in responder-stimulator combinations incompatible for only one HLA-B antigen, were used to study the specificity and genetics of target cell determinants. Specific CTLs directed against HLA-B7, B8, B27 and Bw44 were developed, as defined by standard serological reagents. CTL-anti-B5 and CTL-anti-Bw35 gave false positive reactions. Specific cytotoxic effector cells can be generated against B27 by sensitization of cells from a B7 donor and vice versa; B7 and B27 can in this way be recognized easily. The data support the view that HLA-B is the target antigen. Population and family studies revealed that extra reactions with CTLs developed in female (parous) responder-male stimulator combinations were due to HLA-restricted H-Y antigen killing. By selecting responder-stimulator pairs differing for only 1 antigen at a locus, it should be possible to develop CTLs which will allow recognition of other specificities at the HLA-B locus and other loci.
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