Induction of Prophages of Enterohemorrhagic Escherichia coli O157:H7 with Norfloxacin
- 1 April 1999
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 181 (7) , 2257-2260
- https://doi.org/10.1128/jb.181.7.2257-2260.1999
Abstract
Norfloxacin (NFLX) caused induction of prophages VT1 and VT2 of enterohemorrhagic Escherichia coli O157 at subinhibitory concentrations. In time course experiments, we observed the following sequential events: upon induction, the phage genomes underwent multiplication; the amount of stx genes increased; and subsequently, large quantities of toxins VT1 and VT2 were produced. Further studies showed that the molecular mechanism of prophage induction is closely related to the RecA system since the prophage VT2 was not induced with NFLX in a recA mutant strain.Keywords
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