Transgenic Tobacco plants with T-DNA Phytohormone synthesis genes
- 1 January 1991
- journal article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 33 (1) , 40-48
- https://doi.org/10.1007/bf02873786
Abstract
Agrobacterium tumefaciens binary vectors carrying kanamycin resistance gene and either C58 T-DNA gene 4 for cytokinin synthesis or genes 1 and 2 for auxin synthesis were constructed and used for transformation of a short-day tobacco Maryland Mammoth. Kanamycin resistant plants were regenerated from a small fraction of transformed tissue and the presence of T-DNA in their genome was verified by Southern blotting. The level of endogenous cytokinin in plants transgenic for gene 4 and the level of endogenous IAA in those transgenic for genes 1 and 2 increased by more than 100 %. A number of morphological characteristics distinguish them from untransformed controls.Keywords
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