Changes in Intracellular Free Magnesium During Hypoglycaemia and Hypoxia in Cerebral Tissue as Calculated from31p-Nuclear Magnetic Resonance Spectra
- 1 August 1989
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 53 (2) , 331-334
- https://doi.org/10.1111/j.1471-4159.1989.tb07338.x
Abstract
31P-nuclear magnetic resonance spectra of superfused cerebral tissues were obtained under normal, hypoglycaemic and hypoxic conditions. Concentrations of free intracellular magnesium were calculated from differences in chemical shifts between the .alpha.- and .beta.-resonances of the nucleoside phosphates. Control levels of 0.33 mM were significant increased to 0.52 mM in hypoglycaemia and to 0.57 mM in severe hypoxia. Removal of calcium from the superfusing medium increased the free intracellular Mg2+ concentration to 0.63 mM.Keywords
This publication has 27 references indexed in Scilit:
- Effects of Hypoglycaemia and Hypoxia on the Intracellular pH of Cerebral Tissue as Measured by 31P Nuclear Magnetic ResonanceJournal of Neurochemistry, 1989
- Ca2+ transient, Mg2+, and pH measurements in the cardiac cycle by 19F NMR.Proceedings of the National Academy of Sciences, 1988
- Measurement of Free Intracellular Calcium in the Brain by 19F‐Nuclear Magnetic Resonance SpectroscopyJournal of Neurochemistry, 1988
- Measurement of cytosolic free magnesium ion concentration by fluorine-19 NMRBiochemistry, 1988
- Intracellular free magnesium in synaptosomes measured with entrapped eriochrome blueBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- 31P nuclear-magnetic-resonance studies on superfused cerebral tissuesBiochemical Society Transactions, 1985
- 31P-n.m.r. studies on cerebral energy metabolism under conditions of hypoglycaemia and hypoxia in vitroBiochemical Journal, 1983
- Ionized magnesium concentration in axoplasm of dialyzed squid axonsFEBS Letters, 1975
- Adenine nucleotides and magnesium ions in relation to control of mammalian cerebral-cortex hexokinaseBiochemical Journal, 1969
- Nuclear Magnetic Resonance Spectra of Adenosine Di- and TriphosphateJournal of Biological Chemistry, 1962