Involvement of intracellular Ca2+ in oxidant‐induced NF‐κB activation

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Abstract
In human Jurkat T cells and its subclone Wurzburg cells oxidant challenge elevated [Ca2+]i by mobilizing Ca2+ from intracellular stores. In Jurkat cells this effect was rapid and transient, but in Wurzburg cells the response was slow and sustained. H202‐induced NF‐κB activation in Wurzburg cells was not influenced by the presence of extracellular EGTA but was totally inhibited in cells that were loaded with esterified EGTA. In Jurkat cells that are not sensitive to H202‐induced NF‐κB activation, H202 potentiated NF‐κB activation in the presence of sustained high [Ca2+]i following thapsigargin treatment. NF‐κB regulatory effect of α‐lipoate and N‐acetylcysteine appeared to be, at least in part, due to their ability to stabilize elevation of [Ca2+]i following oxidant challenge. Results of this study indicate that a sustained elevated [Ca2+]i is a significant factor in oxidant‐induced NF‐κB activation.