Formation of super-reduced Chromatium high-potential iron–sulphur protein in aqueous solution by pulse radiolysis
- 1 September 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 189 (3) , 641-644
- https://doi.org/10.1042/bj1890641
Abstract
Both the oxidized and reduced forms of Hipip (high-potential iron–sulphur protein) are reduced (approx. 30% yields) by eaq.- in a single-stage process, rate constants 1.7 × 10(10) and 1.8 × 10(10) M-1 . s-1 respectively, at 25 degrees C, pH 7.0 (5 mM-phosphate). Super-reduced Hipip, which is formed in the latter case, has a spectrum which closely resembles that of reduced ferredoxin, i.e. Fe4S4 (SR)4(3-) clusters. The spectrum is stable over 2 s periods investigated. Super-reduced Hipip is reoxidized with O2, rate constant 4.8 × 10(6) M-1 . s-1 at 25 degrees C.This publication has 11 references indexed in Scilit:
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