Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum

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Abstract
In addition to the virus-coded large-T [tumor] and small-t antigens, 2 new classes of proteins were immunoprecipitated by anti SV-40 tumor serum from extracts of various SV-40-transformed [human and mammalian] cell lines. These were as follows: proteins (termed super-T proteins) with MW higher than that of large-T antigen (86,000), which were found in many SV-40-transformed cell lines derived from mouse and rat cells (super-T proteins and large-T antigen appeared to have closely related structures as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides); proteins (termed 55K proteins) with MW ranging from 50,000-60,000, which were present in all SV-40-transformed cell lines examined so far, including those obtained by chromosome-mediated gene transfer. The 55K proteins were not structurally related to large-T antigen, as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides. The data showed that the 55K proteins are largely or totally cell coded.