Abstract
Crossing and progeny analyses showed that 5 deeply pigmented hypovirulent strains contain single nuclear genes that confer the morphology characteristic of a class of European hypovirulent strains formerly called Jaune Regenere or Pigmentato. These single nuclear genes also determine restricted virulence in host chestnut [Castanea dentata] trees. Such strains arise only from ds[double standard]RNA-containing strains and may be the result of integration of cytoplasmic genetic material into the nuclear genome.

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