Experimental Immunization with a Monoclonal Anti-Idiotope Antibody that Mimics the Neisseria gonorrhoeae Lipooligosaccharide Epitope 2C7

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Abstract
An anti-idiotope monoclonal antibody (MAb), called CAl (Ab2), was produced in mice against MAb 2C7, which recognizes a widely in vivo-expressed gonococcallipooligosaccharide (LOS) epitope. Mice immunized with MAb CAl initially had a 2.S-fold increase in IgG (12-fold after a booster) but no increase in IgM anti-LOS (Abl') antibody. Control mice immunized with LOS had a 4.5fold rise in IgG and 4-fold rise in IgM anti-LOS antibody. In rabbits, MAb CAl elicited a 9-fold rise in IgG and a 3.3-fold rise in IgM anti-LOS (Abl') antibody. Abl' antibody bactericidal activity was 1-2 logs greater than that produced by immunization with LOS. Abl' mediated complete human polymorphonuclear leukocyte phagocytosis of 2C7 epitope-positive (but not 2C7 epitopenegative) gonococci. MAb CAl acts as a molecular surrogate (Ab2β) for the nominal LOS antigen and may form the basis for vaccine candidates for human immunization against Neisseria gonorrhoeae.

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