Transforming growth factorβ1 induces differentiation‐specific gene expression in fetal rat brown adipocytes
- 8 May 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 364 (2) , 193-197
- https://doi.org/10.1016/0014-5793(95)00385-m
Abstract
Fetal rat brown adipocytes show a low number of transforming growth factorβ1 (TGF-β1) binding sites of high affinity, revealing the presence of type I, II and IIITGF-β1 receptors and a minor-labeled species of approximately 140 kDa. The culture of cells in the presence ofTGF-β1 induced the expression of the tissue-specific gene uncoupling protein in a dose- and time-dependent manner. In addition,TGF-β1 up-regulates the expression of genes involved in adipogenesis such as fatty acid synthase, glycerol 3-phosphate dehydrogenase, malic enzyme and glucose 6-phosphate dehydrogenase, as well as induces the expression of fibronectin (specific target gene forTGF-β1). Our results suggest thatTGF-β1 is a major signal involved in initiating and/or maintaining the thermogenic and adipogenic differentiation of rat fetal brown adipocytesKeywords
This publication has 34 references indexed in Scilit:
- Mechanism of activation of the TGF-β receptorNature, 1994
- IGF-I Induces the Uncoupling Protein Gene Expression in Fetal Rat Brown Adipocyte Primary Cultures: Role of C/EBP Transcription FactorsBiochemical and Biophysical Research Communications, 1994
- Triiodothyronine induces the expression of the uncoupling protein in long term fetal rat brown adipocyte primary cultures: role of nuclear thyroid hormone receptor expressionEndocrinology, 1994
- Rabbit Articular Chondrocytes (RAC) Express Distinct Transforming Growth Factor-β Receptor Phenotypes as a Function of Cell Cycle PhasesExperimental Cell Research, 1993
- Proliferation of fetal brown adipocyte primary cultures: Relationship with the genetic expression of glucose 6 phosphate dehydrogenaseExperimental Cell Research, 1991
- The Transforming Growth Factor-beta FamilyAnnual Review of Cell Biology, 1990
- TGF-β stimulation and inhibition of cell proliferation: New mechanistic insightsCell, 1990
- Development of the uncoupling protein in the rat brown‐adipose tissue during the perinatal periodEuropean Journal of Biochemistry, 1990
- Hormonal regulation of malic enzyme expression in primary cultures of foetal brown adipocytesBiochemical and Biophysical Research Communications, 1989
- Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probesCell, 1980