Nitrate reductase inactivating factor from rice cells in suspension culture

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Abstract
With a crude extract of cultured rice cells, there was no direct relationship between the activity of NADH nitrate reductase measured and the amount of cell extract used, when the amount was large. It appeared that some factor in the cell extract inactivated nitrate reductase. The inactivating factor could be separated from nitrate reductase by (NH4)2SO4 precipitation. The factor seemed to be protein: 1) it was precipitable with (NH4)2SO4 heat labile, and pronase treatment caused loss of activity; 2) cycloheximide reduced the formation of the inactivating factor. The activity of this factor fluctuated during the growth period. The existence of this inactivating factor was further investigated in various other cultured cells.

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