CHANGES IN LEVELS OF SOME CHEMICAL CONSTITUENTS OF SOYBEAN LEAVES FOLLOWING LEAF SPOT INFECTION

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Abstract
Leaf pigment, nutrient mineral, carbohydrate, and protein level were determined in leaves of soybean, Soja max (L.) Piper var. Harosoy, infected by bacterial blight, Pseudomonas glycinea (Coerper) Stapp, brown spot, Septoria glycines Hemmi, and frogeye, Cercospora sojina Hara. The plants were grown under controlled environmental conditions. The use of colorimetric methods of analysis showed that loss of chlorophyll, with increase in carotenoids, was associated with the intense yellowing symptom of infected leaf tissue when both Ps. glycinea and S. glycines were active in a synergistic manner. Of the major elements, the levels of phosphorus and potassium were increased significantly by S. glycines infection. Of the minor elements, the level of copper was increased to 150, 400, and 750% of the control following infection by C. sojina, S. glycines, and Ps. glycinea, respectively. Among the carbohydrates, high levels of reducing sugars were found following S. glycines infection. The use of starch-gel electrophoresis and fluoroscopy showed that two compounds were absent and four new components were evident in S. glycines-infected leaves. Electrophoresis also revealed that eight fluorescent compounds were common to tissue infected by Ps. glycinea and C. sojina. A marked esterase activity was found in S. glycines-infected leaves.

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