NMR study of self‐paired parallel duplex of d(AAAAACCCCC) in solution
- 20 July 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 306 (2-3) , 223-228
- https://doi.org/10.1016/0014-5793(92)81005-7
Abstract
The oligonucleotide d(A5C5) in solution forms a parallel self‐duplex at neutral and low pH values. H2O NMR spectra at pH 5.1 indicate the presence of five imino resonances at lower temperatures; and the structure is stable up to 60°C. These signals ran arise only from the hemiprotonated C+·C pairs [Westhof, E. and Sundaralingham, M. (1980) Biochemistry 77, 1852–1856; Westhof, E. and Sundaralingham, M. (1980) J. Mol. Biol. 142, 331–361] and constitute the first direct observation of C+·C hemiprotonated pairs in solution. The cross peaks from Hl's and more than five distinct AH8's in 500 MHz 1H 2D‐NOESY spectra indicate that there are two conformationally different and energetically similar A‐tracts. There is good qualitative agreement between NOESY data and two theoretically derived structures in which A‐tracts are reverse Watson‐Crick and reverse Hoogsteen base‐paired, respectively.Keywords
This publication has 14 references indexed in Scilit:
- Visualisation of a 2′-5′ parallel stranded double helix at atomic resolution: crystal structure of cytidylyl-2′,5′-adenosineNucleic Acids Research, 1991
- An unusual DMA structure detected in a telomeric sequence under superhelical stress and at low pHNature, 1989
- Crystal and Molecular Structure of the Sodium Salt of the Dinucleotide Duplex d(CpG)Journal of Biomolecular Structure and Dynamics, 1987
- An all atom force field for simulations of proteins and nucleic acidsJournal of Computational Chemistry, 1986
- A new force field for molecular mechanical simulation of nucleic acids and proteinsJournal of the American Chemical Society, 1984
- Self base pairing in a complementary deoxydinucleoside monophosphate duplex: crystal and molecular structure of deoxycytidylyl-(3'-5')-deoxyguanosineBiochemistry, 1983
- Synthesis of deoxyoligonucleotides on a polymer supportJournal of the American Chemical Society, 1981
- Crystallographic studies of drug-nucleic acid interactions: Proflavine intercalation between the non-complementary base-pairs of cytidilyl-3′,5′-adenosineJournal of Molecular Biology, 1980
- Molecular Structure of Helical Polycytidylic AcidNature, 1963
- The molecular structure of polyadenylic acidJournal of Molecular Biology, 1961