Effects of fasting and feeding on uridine-3-H incorporation into RNA by pancreas slices

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Abstract
From published reports it is uncertain whether changes in ribonucleic aicd content or rates of RNA synthesis are associated with changes i rates of protein synthesis by the pancreas. For this study pigeons have been used which were fed ad lib., fasted 72 hr., or fasted 72 hr. but allowed to feed for 2 hr. prior to the time of study. Pancreatic tissue slices were prepared and icubated for different time periods in 5 ml tissue culture medium, pH 7.4, which contained 1.23 m[mu]moles uridine-3H (UL). RNA and DNA were separated by modifications of methods described by Schmidt -Thannhauser-Schneider. Pancreatic tissue slices obtained from fed pigeons had greater amounts of RNA as well as greater rates of uridine-3H incorporation into RNA than similar slices obtained from fasted controls. Within 2 hr. of feeding previously fasted birds there was a significant increase in uridine-3H icorporation into RNA, whereas RNA content remained unchanged.

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