Imaging of Ca^2+ intracellular dynamics with a third-harmonic generation microscope

We describe the promising development of third-harmonic generation (THG) in laser scanning microscopy for study of the functional imaging of live biological cells. The dynamics of ${\mathrm{Ca}}^{2+}$ in biological cells is shown. The ${\mathrm{Ca}}^{2+}$ signal consists of a transient increase in the intracellular concentration. THG microscopy allows one to temporally visualize the release of ${\mathrm{Ca}}^{2+}$ from internal stores and (or) calcium influx.