Changes in the amount and composition of fractions from extracellular polysaccharide during the culture cycle of cotyledon cell suspension culture of bush bean (Phaseolus vulgaris cv. Contender)

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Abstract
Three different polysaccharides (cold-water-soluble pectin, EDTA-soluble pectin, and neutral polysaccharides) were separated from extracellular polysaccharide produced by bush-bean cotyledon suspension cultures. The different polysaccharides were associated with different phases of the culture cycle. The neutral sugars present in the pectin fractions showed considerable fluctuation during the culture cycle as compared with the rather small changes in the levels of the sugars present in the neutral polysaccharides.

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