An alpha-like DNA polymerase from Halobacterium halobium
- 1 October 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 152 (2) , 293-297
- https://doi.org/10.1111/j.1432-1033.1985.tb09197.x
Abstract
Two DNA-polymerases have been isolated from extracts of Halobacterium halobium one having a sedimentation coefficient of 11 S, designated as .alpha.-like polymerase and possessing the following characteristics. It is sensitive to both aphidicolin and N-ethylmaleimide but indifferent to the presence of a dideoxynucleoside triphosphate. Therefore this polymerase is very similar to the .alpha. DNA polymerase of eukaryotes. The enzyme requires 5 M NaCl for maximum activity. The other polymerase has a sedimentation coefficient of 4.4 S and is resistant to both aphidicolin and N-ethylmaleimide. However, it is inhibited by a dideoxynucleoside triphosphate.Keywords
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