Differential Cytotoxicity of Tumorigenic and Nontumorigenic Strain-2 Guinea Pig Cells as Mediated by Syngeneic Phytohemagglutinin-Stimulated Peritoneal Exudate Cells
- 1 March 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 54 (3) , 743-747
- https://doi.org/10.1093/jnci/54.3.743
Abstract
Phytohemagglutinin (PHA)-stimulated peritoneal exudate (PE) cells from strain-2 guinea pigs were more cytotoxic in culture to syngeneic tumorigenic cells than to syngeneic nontumorigenic cells. Cytotoxicity was measured by the release of 3H-thymidine from prelabeled target cells. Tumor-producing guinea pig fetal cells transformed in culture by chemical carcinogen released up to 70% of their label in the presence of PHAstimulated PE cells. Non-tumor-producing cells, regardless of their morphologic characteristics, were less affected by PHA-stimulated PE cells. Nontumorigenic cultures included untreated early passage and long-term cultures previously treated with a carcinogenic or noncarcinogenic chemical. Differential cytotoxicity to tumorigenic versus nontumorigenic cells was dependent upon both PE cell and PHA concentration. Tumorigenic cultures were best distinguished from nontumorigenic cultures when 0.51–1×106 PE cells and 50–100 µug PHA were incubated with 1×1043H-thymidine-labeled target cells for 48 hours.Keywords
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