Isolation and Characterization of a Monosialosylgangliopentaosyl Ceramide from Xenopus laevis Oocyte1

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Abstract
A monosialosylgangliopentaosyl ceramide was isolated from Xenopus laevis oocytes. It represented 5.8% of the total acidic glycosphingolipids. From the results of sugarcomposition analysis, enzymatic hydrolysis, permethylation analysis, and negative ion fast atom bombardment mass spectrometry, the structure of the ganglioside was determined to be as follows: The predominant species of fatty acids were α-hydroxy fatty acids, h22 : 0, h24 : 0, and h24 : 1. The long chain bases of this ganglioside consisted mainly of dl8 : 1 sphingosine and phytosphingosine. Other acidic glycolipids were also characterized. The most abundant component of acidic glycolipids was sulfatide, which represented 86.7% of the total acidic glycolipid mixture. GM3, GM2, GMla, and GDla were also detected.

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