Effects of GTP,GDP[βS] and glucose on adenylate cyclase activity of E. coli B
- 25 March 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 182 (2) , 429-434
- https://doi.org/10.1016/0014-5793(85)80348-3
Abstract
Adenylate cyclase activity was measured in suspensions of E. coli B, rendered permeable with toluene. The enzyme was activated in a dose‐dependent manner by GTP and by its non‐hydrolysable analogue, GTP[γS]. In contrast, incubation with GDP[βS], a non‐phosphorylatable analogue of GDP, caused a dose‐related inhibition of adenylate cyclase; this was partially overcome by addition of GTP. GTP did not relieve, and GDP[βS] augmented, the non‐competitive and dose‐related inhibition of E.coli adenylate cyclase by glucose.Keywords
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