Association of deoxyribonuclease-sensitive material with adenovirus penton aggregates after treatment of infected cell cultures with sodium deoxycholate
- 1 August 1971
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 17 (8) , 1009-1013
- https://doi.org/10.1139/m71-161
Abstract
Sodium deoxycholate treatment of adenovirus-infected cell cultures was found to release rapidly sedimenting penton components with a buoyant density in CsCl of about 1.34 g/ml. The high-density penton material derived from human and simian adenovirus-infected cells occurred in monomeric form, while that derived from canine adenovirus-infected cells was in the form of non-symmetrical groupings of pentons aggregated at the vertex capsomere. Deoxyribonuclease treatment and 3H-thymidine-DNA labeling experiments revealed that the high-density of the pentons so obtained was due to a small associated segment of nuclease-sensitive material. A possible explanation of the nature of the nuclease-sensitive material is given.Keywords
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