Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators
- 15 September 1999
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 13 (18) , 2369-2374
- https://doi.org/10.1101/gad.13.18.2369
Abstract
SWI/SNF is a chromatin remodeling complex that facilitates expression of a number of yeast genes. Here we demonstrate that SWI/SNF can be recruited from yeast nuclear extracts by a transcriptional activator. Recruitment is dependent on an activation domain but not on promoter sequences, TBP, or RNA polymerase II holoenzyme. We also show that acidic activation domains can target SWI/SNF remodeling activity. These results demonstrate that SWI/SNF activity can be targeted by gene-specific activators and that this recruitment can occur independently of Pol II holoenzyme.Keywords
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