Root-Tip Smear Method for Difficult Material
- 1 January 1939
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 14 (4) , 147-149
- https://doi.org/10.3109/10520293909110318
Abstract
A technic is outlined for the preparation of difficult material for the study of chromosome number and morphology in root-tip smears. The chief objective is to obtain polar views of the metaphase plates rather than equatorial views. To achieve this end it is recommended that fresh root-tips be cut free-hand into thin cross sections. The important features are: thin freehand cross sectioning of the fresh root-tips; fixing in Belling's iron aceto-carmine solution; maceration for 2–5 minutes in 50% HCl in 95% alcohol; and mounting in “Diaphane.”Keywords
This publication has 1 reference indexed in Scilit:
- A PERMANENT ROOT TIP SMEAR METHODStain Technology, 1935