Kinetic Determination of Glucose with the GEMSAEC (ENI) Centrifugal Analyzer by the Glucose Dehydrogenase Reaction, and Comparison with Two Commonly Used Procedures

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Abstract
A new glucose dehydrogenase preparation has been used to determine the glucose concentration in serum or plasma with the GEMSAEC(ENI) analyzer. The reaction is sufficiently linear to be suitable for a kinetic determination. A sample volume of 15 µl or less is needed, and 14 determinations can be done simultaneously within 160 s (the time needed for loading the samples and reagents into the distribution disc plus a reaction time of 70 s). The reaction is linear up to 300 mg of glucose per 100 ml, but with special computer software linearity could be extended to 1 g of glucose per 100 ml. Day-to-day and within-day precision have been tested with a number of different sera and standards. Accuracy has been checked by comparing the results with those obtained by the hexokinase and the glucose oxidase/peroxidase methods. A better agreement was found with the hexokinase method. The proposed procedure has the advantage of involving only one enzymatic step and of directly measuring reduced coenzyme formation at 340 nm.

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