Identification of β Vartant Hemoglobins by DNA Restriction Endonuclease Mapping
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 8 (5) , 443-462
- https://doi.org/10.3109/03630268408991731
Abstract
An alternative method for identifying β variant hemoglobins is described. Computer analysis of restriction sites was used to predict which β variants could be detected by DNA mapping. 61 of 217 variants were shown to have changes in restriction fragment patterns which were useful markers for the abnormal hemoglobin. A further 25 could be identified by polyacrylamide electrophoresis. Implications of DNA analysis in diagnosis of variant hemoglobins are discussed.Keywords
This publication has 19 references indexed in Scilit:
- Analysis of the Hb M Milwaukee mutation at the DNA levelBritish Journal of Haematology, 1983
- The bacteriopbage λ O replication protein: isolation and characterization of the amplified initiatorNucleic Acids Research, 1983
- Improved Detection of the Sickle Mutation by DNA AnalysisNew England Journal of Medicine, 1982
- ANTENATAL DIAGNOSIS OF SICKLE CELL ANAEMIA BY DIRECT ANALYSIS OF THE SICKLE MUTATIONThe Lancet, 1981
- DNA analysis and the antenatal diagnosis of hemoglobinopathiesPublished by Elsevier ,1981
- The nucleotide sequence of the human β-globin geneCell, 1980
- The detection and use of hemoglobin mutants in the direct analysis of human globin genesBlood, 1980
- γ-β-Thalassaemia studies showing that deletion of the γ- and δ-genes influences β-globin gene expression in manNature, 1980
- Polymorphism of DNA Sequence in the β-Globin Gene RegionNew England Journal of Medicine, 1980
- Nucleotide Sequence Specificity of Restriction EndonucleasesScience, 1979