LETHAL EFFECTS OF 1-BETA-D-ARABINOFURANOSYLCYTOSINE INCORPORATION INTO DEOXYRIBONUCLEIC-ACID DURING ULTRAVIOLET REPAIR

  • 1 January 1984
    • journal article
    • research article
    • Vol. 25  (2) , 322-326
Abstract
1-.beta.-D-Arabinofuranosylcytosine (ara-C) incorporates into leukemic cell DNA and the extent of this incorporation correlates significantly with loss of clonogenic survival. Studies were extended by monitoring the incorporation of ara-C in DNA undergoing repair of UV-induced damage. The studies were performed using human foreskin diploid fibroblast that undergo density-dependent growth arrest. Ara-C incorporates specifically into DNA undergoing repair of damage by UV light. The extent of ara-C incorporation in AG1522 DNA during UV repair correlates significantly (P < 0.0006) with cell lethality. The findings confirm that incorporation of Ara-C into DNA undergoing either semiconservative or unscheduled DNA synthesis results in lethal cellular events. The finding may provide the experimental basis for the development of new therapeutic approaches using ara-C in combination with agents that damage DNA.

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