Glucose Metabolism in the Normal Anesthetized Dog Injected Successively with Anti-insulin Serum and Insulin

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Abstract
Seven fasted, healthy, anesthetized dogs were successively injected at a 2-hr. interval with guinea pig anti-insulin serum and with insulin. The dose of anti-insulin serum used had a total neutralizing potency of 2 U. insulin/kg body weight; the amount of insulin used was sufficient to restore normal blood glucose level. Glucose production was determined by hepatic catheterization; glucose tissue utilization by the study of the disappearance rate of a tracer dose of glucose l-C-14. Anti-insulin serum induced hyperglycemia in 5 dogs and failed to provoke any change in blood glucose among the 2 others. This difference of behavior was probably related with differing sensitivity to the same fast. In responders, hyperglycemia was accompanied by an increase in liver glucose output and by a reduction of tissue glucose utilization; insulin, when given in sufficient amount to restore normal blood glucose level, induced a decrease of glucose production and an increase in glucose utilization to basal values. In the nonresponders, together with a slight decrease in glucose utilization, a tendency to reduced glucose production was noticed. The doses of insulin necessary to provoke hypoglycemia were 3 times more important than those used in the responders.