Serum Can Act as a Shear Protecting Agent in Agitated Hybridoma Cell Cultures
- 1 December 1989
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 8 (6) , 639-645
- https://doi.org/10.1089/hyb.1989.8.639
Abstract
Hybridoma cells, S3H5/γ2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.Keywords
This publication has 4 references indexed in Scilit:
- Effect of mechanical agitation on hybridoma cell growthBiotechnology Letters, 1988
- Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentrationJournal of Biotechnology, 1988
- Evidence that monoclonal antibody production kinetics is related to the integral of the viable cells curve in batch systemsBiotechnology Letters, 1988
- Transient responses of hybridoma metabolism to changes in the oxygen supply rate in continuous cultureBioprocess and Biosystems Engineering, 1988