Determination of Dissolved Hydrogen Concentration and in Submerged Culture Vessels

Abstract
The determination of dissolved hydrogen concentration in the culture medium of hydrogen bacteria was performed. Dissolved hydrogen in the medium was stripped by bubbling nitrogen and thus stripped hydrogen was quantitatively determined by a gas Chromatograph. values similar to Bunsen’s absorption coefficients were observed by this method. In addition, volumetric hydrogen transfer coefficients, were calculated under various conditions based on the observed values. values thus obtained were found to be similar to the estimated values according to Wilke’s equation and values.

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