Generation and Characterization of two Monoclonal Self‐Associating IgG Rheumatoid Factors from a Rheumatoid Synovium
Open Access
- 1 January 1992
- journal article
- research article
- Published by Wiley in Arthritis & Rheumatism
- Vol. 35 (1) , 101-105
- https://doi.org/10.1002/art.1780350115
Abstract
Objective. To study IgG rheumatoid factor (RF) from rheumatoid synovium.Methods. We fused the K6H6/B5 human—mouse heterohybridoma with unstimulated rheumatoid synovial B cells to generate IgG‐RF—secreting hybridomas.Results. The RFs from 2 such hybridomas bound specifically to the Fc fragment of human IgG and self‐associated to form immune complexes. Such immune complexes are a major characteristic of the pathogenic IgG‐RFs in rheumatoid synovium.Conclusion. IgG‐RF—secreting hybridomas have been obtained. Analyses may reveal the underlying mechanisms of the induction of IgG‐RF.Keywords
This publication has 11 references indexed in Scilit:
- The complete nucleotide sequences of the heavy chain variable regions of six monospecific rheumatoid factors derived from Epstein-Barr virus-transformed B cells isolated from the synovial tissue of patients with rheumatoid arthritis. Further evidence that some autoantibodies are unmutated copies of germ line genes.Journal of Clinical Investigation, 1990
- Serologic and molecular characterization of a human monoclonal rheumatoid factor derived from rheumatoid synovial cellsArthritis & Rheumatism, 1990
- From Human Autoantibodies to the Fetal Antibody Repertoire to B Cell Malignancy: It's a Small World After AllInternational Reviews of Immunology, 1990
- Probing the Human B-Cell Repertoire with EBV: Polyreactive Antibodies and CD5+ B LymphocytesAnnual Review of Immunology, 1989
- Rheumatoid factors in immune complexes of patients with rheumatoid arthritisSpringer Seminars in Immunopathology, 1988
- The american rheumatism association 1987 revised criteria for the classification of rheumatoid arthritisArthritis & Rheumatism, 1988
- Human monoclonal IgG rheumatoid factor has structural homology with bacterial Fc receptor proteins.The Journal of Immunology, 1987
- Mouse × human heterohybridomas as fusion partners with human B cell tumorsJournal of Immunological Methods, 1986
- Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assayJournal of Immunological Methods, 1985
- The Immunopathology of Joint Inflammation in Rheumatoid ArthritisPublished by Elsevier ,1973