NMR Conformational Analysis of DNA Duplexes Containing Diol Epoxide Adducts of Polycyclic Aromatic Hydrocarbons
- 1 November 1996
- journal article
- research article
- Published by Taylor & Francis in Polycyclic Aromatic Compounds
- Vol. 10 (1-4) , 145-152
- https://doi.org/10.1080/10406639608034691
Abstract
The principal adducts formed between DNA and polycyclic aromatic hydrocarbon diol epoxides result from N-alkylation of the exocyclic amino groups of the purine bases by the benzylic carbon atom of the epoxide. To date, the solution conformations of more than a dozen alkylated DNA duplexes have been examined by 2D NMR spectroscopy. For trans opened diol epoxides, oligomer duplexes containing N2-adducts at deoxyguanosine have the hydrocarbon residue lying in the minor groove whereas those containing N6-adducts at deoxyadenosine have the hydrocarbon intercalated within the DNA helix. Absolute configuration at the site of attachment appears to be a major determinant in establishing whether the hydrocarbon lies to the 3′- or the 5′-side of the adducted base. For trans opened deoxyadenosine adducts with R-absolute configuration, the hydrocarbon residue is positioned toward the 5′-end of the adducted strand whereas trans opened deoxyguanosine adducts with R-absolute configuration have the hydrocarbon located toward the 3′-end of the adducted strand.Keywords
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