An inverse relationship between receptor internalization and the fraction of laterally mobile receptors for the vasopressin renal‐type V2‐receptor
- 12 November 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 274 (1-2) , 223-226
- https://doi.org/10.1016/0014-5793(90)81368-x
Abstract
Lateral mobility of the vasopressin renal‐type V2‐receptor was investigated in LLC‐PK1, porcine epithelial cells using the technique of fluorescence microphotolysis (photobleaching) and a rhodamine‐labelled vasopressin analogue. At various times after ligand addition, cells were analyzed for both receptor lateral mobility and ligand internalization. The V2‐receptor mobile fraction diminished from 0.9 to 0.43 over 60 min at 37°C. whereas the apparent lateral diffusion coefficient remained essentially unchanged (2–3 × 10−10cm2s). Interestingly, the fraction of immobile V2‐receptors corresponded exactly with the fraction of internalized receptors, implying a functional relationship. These observations together with comparable results reported for other polypeptide hormone receptors indicate a possible machanistic role for receptor immobilization in the desensitization of hormonal response.Keywords
This publication has 31 references indexed in Scilit:
- Lateral mobility of proteins and lipids in the red cell membrane and the activation of adenylate cyclase by β‐adrenergic receptorsFEBS Letters, 1988
- GTP analogues cause release of the alpha subunit of the GTP binding protein, GO, from the plasma membrane of NG108-15 cellsBiochemical and Biophysical Research Communications, 1988
- Self-phosphorylation of epidermal growth factor receptor: evidence for a model of intermolecular allosteric activationBiochemistry, 1987
- The G protein connection: is it in the membrane or the cytoplasm?Trends in Biochemical Sciences, 1987
- Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobilityBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1986
- α‐Subunits of Ns are released from the plasma membrane following cholera toxin activationFEBS Letters, 1986
- Vasopressin induced production of inositol trisphosphate and calcium efflux in a smooth muscle cell lineBiochemical and Biophysical Research Communications, 1985
- Lateral diffusion of epidermal growth factor complexed to its surface receptors does not account for the thermal sensitivity of patch formation and endocytosisBiochemistry, 1982
- A microfluorimetric study of translational diffusion in erythrocyte membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- Membrane ReceptorsAnnual Review of Biochemistry, 1974