Improved Cell Dispersal Method by Low-Temperature Incubation of Tissue in a Trypsin-Citrate Solution

Open Access

1 January 1972

journal article
research article
Published by American Society for Microbiology in Applied Microbiology

Vol. 24 (3) , 506-507
https://doi.org/10.1128/aem.24.3.506-507.1972

Abstract

Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.

Keywords

REMOVE
TISSUE
VIABLE
WASHED
PROCEDURES
DISRUPTED
LOW TEMPERATURE
INCUBATION
GAVE
SOAKED