Photoreduction of NADP+ by a chloroplast photosystem II preparation: effect of light intensity
- 14 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 253 (1-2) , 253-256
- https://doi.org/10.1016/0014-5793(89)80970-6
Abstract
Recent work in this and other laboratories has demonstrated that, contrary to the favored Z scheme hypothesis, photosystem II (PS II) can photoinduce electron transfer from water to NADP+, without the participation of photosystem I (PS I). One proposed explanation for this conflict between hypothesis and observation was that PS II can reduce NADP+ but only at high light intensities. We report here findings at variance with this proposal. A PS II preparation made from spinach chloroplasts by the two‐phase aqueous polymer partition method photoreduced NADP+ without the involvement of PS I, at varying light intensities ranging from limiting to saturating.Keywords
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