CHIMERISM IN SKIN OF BONE MARROW TRANSPLANT RECIPIENTS

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Abstract
Skin biopsies from 3 patients receiving 1-haplotype-matched bone marrow grafts have provided a unique opportunity to demonstrate the presence of donor cells in situ using immunohistological techniques and a monoclonal antibody directed against an epitope common to HLA-A2 and HLA-A28 antigens. The infiltrating cells were also analyzed in consecutive tissue sections with a panel of monoclonal antibodies to human leukocyte antigens, T cells and epidermal Langerhans cells. Most of the infiltrating cells were T lymphocytes of donor origin, regardless of whether the histological changes were consistent with graft-vs.-host disease (GVHD) or were eczematous. Donor T cells were also shown to colonize histologically normal skin soon after transplantation. Epidermal keratinocytes, dermal endothelium and adnexal structures did not express the donor HLA type (i.e., were host derived), but the origin of the epidermal Langerhans cells could not clearly be establshed. Donor cells apparently preferentially migrate to certain sites in skin after transplantation and are not always associated with GVHD.