Rapid radioimmunoassay of total urinary estriol.
Open Access
- 1 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 22 (5) , 611-615
- https://doi.org/10.1093/clinchem/22.5.611
Abstract
We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic. Using semi-automatic pipetting equipment, one laboratory technologist can complete 50 assays within 8 h. This technique is both reliable and convenient and should decrease the expense of routine estriol assays.This publication has 0 references indexed in Scilit: