Extragenic suppression of the requirement for diaminopimelate in diaminopimelate auxotrophs ofMycobacterium smegmatis
Open Access
- 1 August 2003
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 225 (1) , 131-135
- https://doi.org/10.1016/s0378-1097(03)00502-0
Abstract
Mycobacteria, like many prokaryotes, have a peptidoglycan with peptides composed of l-alanine (or glycine), d-iso-glutamine, meso-diaminopimelate, and d-alanine. We sought to study mycobacterial peptidoglycan biosynthesis by constructing diaminopimelate (DAP) auxotrophs of Mycobacterium smegmatis and then isolating spontaneous mutants of these auxotrophs that can grow in the absence of DAP. Here we report the isolation and characterization of seven classes of spontaneous M. smegmatis mutants with extragenic mutations that can suppress the DAP requirement of DAP auxotrophs.Keywords
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