Calcitonin receptor binding as a marker of osteoclast heterogeneity in osteopetrotic rodents
Open Access
- 1 December 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Bone and Mineral Research
- Vol. 1 (6) , 543-553
- https://doi.org/10.1002/jbmr.5650010609
Abstract
We have employed a radioautographic technique to examine in vivo receptor binding of calcitonin to osteoclasts in four rodent mutants with osteopetrosis. 125I-Labeled calcitonin was injected intravenously alone or with excess unlabeled calcitonin to osteopetrotic (op/op), osteosclerotic (oc/oc), and microphthalmic (mi/mi) mice and to incisor absent (ia/ia) rats. Similar experiments were performed simultaneously in phenotypically normal littermates. Specific binding of calcitonin to receptors on osteoclasts and osteoclast morphology were then examined by light and electron microscope radioautography. Calcitonin binding was increased in mi/mi mice, where osteoclasts were abundant but reduced in size, and was also increased in op/op mice in association with an undulated and redundant osteoclast cell membrane. Binding of the hormone was markedly diminished on osteoclasts of oc/oc mice and ia/ia rats. Thus, in these rodent models of osteopetrosis all of which manifest reduced skeletal remodeling and share a recessive pattern of inheritance, considerable heterogeneity of osteoclast characteristics was demonstrable. Although calcitonin may play no primary pathogenetic role in most forms of this disease, calcitonin receptor binding is a morphological and functional marker of osteoclasts that can be used in assessing the pathophysiology of disorders of bone remodeling.Keywords
Funding Information
- Medical Research Council of Canada
- National Institute for Dental Research ((DE-07444))
This publication has 38 references indexed in Scilit:
- Fusion disability of embryonic osteoclast precursor cells and macrophages in the microphthalmic osteopetrotic mouseBone, 1985
- Congenital Osteopetrotic Mutations as Probes of the Origin, Structure, and Function of OsteoclastsClinical Orthopaedics and Related Research, 1984
- An interleukin 1 like factor stimulates bone resorption in vitroNature, 1983
- Decreased response to calcitonin in osteopetrotic microphthalmic mouse boneNature, 1979
- Pathogenesis of osteopetrosis in the microphthalmic mouse: Reduced bone resorptionJournal of Anatomy, 1977
- A discrepancy between measurements of bone resorption in vivo and in vitro in newborn osteopetrotic ratsJournal of Anatomy, 1974
- Pathogenesis of osteopetrosis in the ia rat: Reduced bone resorption due to reduced osteoclast functionJournal of Anatomy, 1973
- Calcitonin Receptors of Kidney and BoneScience, 1972
- Ultrastructural effects of calcitonin on osteoclasts in tissue cultureJournal of Ultrastructure Research, 1972