Pyridoxal Compounds as Specific Reagents for the α and β N-Termini of Hemoglobin
- 1 September 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (9) , 2595-2599
- https://doi.org/10.1073/pnas.70.9.2595
Abstract
Bifunctional pyridoxal derivatives with a charged side chain in the 5′ position react specifically with the N-terminal valine residues of hemoglobin. Three of these compounds, which have only a single negative charge in the side chain, are highly selective for the α-chain N-terminal residues in the oxy conformation while pyridoxal phosphate, with two negative charges, reacts specifically with the β N-terminal in deoxyhemoglobin. Schiff's base formation, therefore, results in decreased oxygen affinity with pyridoxal phosphate but increased oxygen affinity with the other three compounds. Reduction with sodium borohydride leads to irreversible coupling at either end of the molecule, and preparation and properties of such derivatives are described.Keywords
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