Enhanced Hepatic Microsomal Activity by Pretreatment of Rats with Acetone or Isopropanol

Abstract
Summary Rat liver microsomes isolated from rats pretreated orally with acetone or isopropanol possessed an enhanced capacity to bind 14CCl4 and 14CHCl3 covalently and to demethylate DMN. The N-demethylation of ethylmorphine was unchanged by this pretreatment. Since there was no increase in the level of microsomal protein, and microsomal cytochrome P-450 or the activity of NADPH-cytochrome c reductase, the mechanism of this enhanced metabolism remains unclear. The authors acknowledge the excellent technical assistance of E. M. Boykins, M. A. Williams and W. Saul.

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