INVASIVENESS OF HYPERPLASTIC NODULE CELLS FROM DIETHYLNITROSAMINE TREATED RAT-LIVER

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (12) , 5162-5175
Abstract
Administration of diethylnitrosamine p.o. [per os] to female Sprague-Dawley rats induced hepatocellular lesions antecedent to hepatocarcinoma (altered foci and hyperplastic nodules). Hepatocytes from hyperplastic nodules were tested for their invasiveness in vitro, which is a marker for malignancy. The hyperplastic nodule cells are compared with control liver cells and the hepatocarcinoma are collected as fragments taken directly from the rat liver. Nodules isolated by collagenase perfusion of the liver, are collected on a filter. The fragments of normal liver, the nodules and the hepatocarcinomas were brought in contact with precultured 9 day old embryonic chick heart fragments for attachment to each other to form confronting cultures. After attachment the confronting cultures were incubated at 37.degree. C on a gyratory shaker for 24 h-14 days. Hepatocytes from the nodules show progressive invasion into the precultured heart fragments in the same way as the hepatocarcinoma cells after 3-14 days in vitro. The control hepatocytes from the liver fragments showed no invasion. The cells of the nodules must be considered as malignant altered hepatocytes since they show progressive invasiveness in vitro.