Aphidicolin as synchronizing agent in root tip meristems of Haplopappus gracilis
- 1 June 1983
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 2 (3) , 156-159
- https://doi.org/10.1007/bf00269343
Abstract
Aphidicolin, a reversible inhibitor of nuclear DNA replication, was tested as syncrhonizing agent in root tip meristems of Haplopappus gracilis. Embryos (i.e. decoated seeds) or 3-day-old seedlings were used to this purpose. After a 24 h treatment with the drug, a high level of synchrony was observed in both experimental materials for two cell cycles as assessed by determining the accumulation of cells in the S and M phases of the cycle. Highest synchronization was obtained with germinating embryos, possibly owing to a low degree of synchrony already existing in this system.Keywords
This publication has 23 references indexed in Scilit:
- Synchronization of nuclear DNA synthesis in cultured Daucus carota L. cells by aphidicolinFEBS Letters, 1983
- DNA repair synthesis in plant protoplasts is aphidicolin‐resistantFEBS Letters, 1982
- Functional roles of the plant α‐like and γ‐like DNA polymerasesFEBS Letters, 1981
- Specific and reversible inhibition by aphidicolin of the α‐like DNA polymerase of plant cellsFEBS Letters, 1980
- Interaction between abscisic acid and fusicoccin during germination and post-germinative growth in Haplopappus gracilisPlant Science Letters, 1979
- Comparative effects of fusicoccin and gibberellic acid on the promotion of germination and DNA synthesis initiation in Haplopappus gracilisPlant Science Letters, 1975
- X-Ray crystallographic determination of the structure of the antibiotic aphidicolin: a tetracyclic diterpenoid containing a new ring systemJournal of the Chemical Society, Chemical Communications, 1972
- Partial Synchronization of Cell Division in Suspension Cultures of Haplopappus gracilisPhysiologia Plantarum, 1966
- High mitotic synchronization induced by 5-aminouracil in root cells of Allium cepa LExperimental Cell Research, 1966
- Differences in mitotic cycle in various tissues of Vicia faba roots as revealed by 5-amino uracil synchronization of cell divisionExperimental Cell Research, 1966