Regulation of lipopolysaccharide‐induced inflammatory response by glutathione S‐transferase P1 in RAW264.7 cells
- 1 July 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (19) , 4081-4087
- https://doi.org/10.1016/j.febslet.2005.06.034
Abstract
Glutathione S‐transferase P1(GSTP1) plays an important role in the detoxification and xenobiotics metabolism. Here, we show that GSTP1 is also involved in LPS (lipopolysaccharide)‐induced inflammatory response. GSTP1 expression, determined at the transcription and translation levels, were upregulated by the LPS stimulation in RAW264.7 macrophage‐like cells. GSTP1 inhibited LPS‐induced mitogen‐activated protein kinases MAPKs including ERK, JNK and p38 as well as NF‐κB activation dose‐ and time‐dependently in transient transfected and stable transfected cells. Moreover this inhibition of the signaling pathways resulted in the decrease of tumor necrosis factor alpha (TNF‐α) and nitric oxide (NO) synthesis. These data suggest that the GSTP1 prevents LPS‐induced excessive production of pro‐inflammatory factors and plays an anti‐inflammatory role in response to LPS.Keywords
This publication has 27 references indexed in Scilit:
- Redox regulation of pro-inflammatory cytokines and IκB-α/NF-κB nuclear translocation and activationBiochemical and Biophysical Research Communications, 2002
- A Genomic and Proteomic Analysis of Activation of the Human Neutrophil by Lipopolysaccharide and Its Mediation by p38 Mitogen-activated Protein KinaseJournal of Biological Chemistry, 2002
- Human Glutathione Transferase P1-1 and Nitric Oxide CarriersJournal of Biological Chemistry, 2001
- Role of redox potential and reactive oxygen species in stress signalingOncogene, 1999
- Induction of Cell Proliferation and Collagen Synthesis in Human Small Intestinal Lamina Propria Fibroblasts by Lipopolysaccharide: Possible Involvement of Nitric OxideBiochemical and Biophysical Research Communications, 1997
- Bacterial infection induces nitric oxide synthase in human neutrophils.Journal of Clinical Investigation, 1997
- Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitin-proteasome pathway.Genes & Development, 1995
- Pro-inflammatory Cytokines and Environmental Stress Cause p38 Mitogen-activated Protein Kinase Activation by Dual Phosphorylation on Tyrosine and ThreonineJournal of Biological Chemistry, 1995
- Function and Activation of NF-kappaB in the Immune SystemAnnual Review of Immunology, 1994
- Passive Immunization Against Cachectin/Tumor Necrosis Factor Protects Mice from Lethal Effect of EndotoxinScience, 1985