Insertional mutagenesis in mice: new perspectives and tools

1 July 2005

journal article
review article
Published by Springer Nature in Nature Reviews Genetics

Vol. 6 (7) , 568-580
https://doi.org/10.1038/nrg1638

Abstract

Insertional mutagenesis has been at the core of functional genomics in many species. In the mouse, improved vectors and methodologies allow easier genome-wide and phenotype-driven insertional mutagenesis screens. The ability to generate homozygous diploid mutations in mouse embryonic stem cells allows prescreening for specific null phenotypes prior to in vivo analysis. In addition, the discovery of active transposable elements in vertebrates, and their development as genetic tools, has led to in vivo forward insertional mutagenesis screens in the mouse. These new technologies will greatly contribute to the speed and ease with which we achieve complete functional annotation of the mouse genome.

Keywords

This publication has 100 references indexed in Scilit:

APOBEC3G cytidine deaminase inhibits retrotransposition of endogenous retroviruses
Nature, 2005
A public gene trap resource for mouse functional genomics
Nature Genetics, 2004
Identification of autonomous IAP LTR retrotransposons mobile in mammalian cells
Nature Genetics, 2004
A resource for large-scale RNA-interference-based screens in mammals
Nature, 2004
A mouse model of human L1 retrotransposition
Nature Genetics, 2002
Conditional control of gene expression in the mouse
Nature Reviews Genetics, 2001
Creation of genome-wide protein expression libraries using random activation of gene expression
Nature Biotechnology, 2001
Functional Annotation of Mouse Genome Sequences
Science, 2001
Stage-specific apoptosis, developmental delay, and embryonic lethality in mice homozygous for a targeted disruption in the murine Bloom’s syndrome gene
Genes & Development, 1998
Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chromsome
Nature Biotechnology, 1997