In Vivo Reporter Labeling of Proteins via Metabolic Delivery of Coenzyme A Analogues
- 23 July 2005
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (32) , 11234-11235
- https://doi.org/10.1021/ja052911k
Abstract
We demonstrate site-specific reporter labeling of proteins within live cells. By accessing the coenzyme A (CoA) metabolic pathway with a cell-permeable pantetheine analogue, we deliver CoA-bound reporter molecules for post-translational protein modification in vivo. These methods may be applied to natural product pathway manipulation as well as applications in conventional molecular and cellular biology.Keywords
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