Feline Foamy Virus Genome and Replication Strategy
Open Access
- 1 November 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (21) , 11324-11331
- https://doi.org/10.1128/jvi.77.21.11324-11331.2003
Abstract
Crucial aspects of the foamy virus (FV) replication strategy have so far only been investigated for the prototypic FV (PFV) isolate, which is supposed to be derived from nonhuman primates. To study whether the unusual features of this replication pathway also apply to more-distantly related FVs, we constructed feline FV (FFV) infectious molecular clones and vectors. It is shown by quantitative RNA and DNA PCR analysis that FFV virions contain more RNA than DNA. Full-length linear DNA was found in extracellular FFV by Southern blot analysis. Similar to PFV, azidothymidine inhibition experiments and the transfection of nucleic acids extracted from extracellular FFV indicated that DNA is the functional relevant FFV genome. Unlike PFV, no evidence was found indicating that FFV recycles its DNA into the nucleus.Keywords
This publication has 53 references indexed in Scilit:
- Retrotransposition and Cell-to-Cell Transfer of Foamy VirusesJournal of Virology, 2003
- Mutation of the Catalytic Domain of the Foamy Virus Reverse Transcriptase Leads to Loss of Processivity and InfectivityJournal of Virology, 2002
- Specific Interaction of a Novel Foamy Virus Env Leader Protein with the N-Terminal Gag DomainJournal of Virology, 2001
- A Particle-Associated Glycoprotein Signal Peptide Essential for Virus Maturation and InfectivityJournal of Virology, 2001
- Nuclear Localization of the Functional Bel 1 Transactivator but Not of the Gag Proteins of the Feline Foamy VirusVirology, 1998
- Cells Expressing the Human Foamy Virus (HFV) Accessory Bet Protein Are Resistant to Productive HFV SuperinfectionVirology, 1998
- Human Foamy Virus Replication: A Pathway Distinct from That of Retroviruses and HepadnavirusesScience, 1996
- Infectious Proviral Clones of Chimpanzee Foamy Virus (SFVcpz) Generated by Long PCR Reveal Close Functional Relatedness to Human Foamy VirusVirology, 1995
- Replicating Foamy Virus-Based Vectors Directing High Level Expression of Foreign GenesVirology, 1995
- Hepatitis B virus replicationTrends in Microbiology, 1993