Determination of LewisFUT3gene mutations by PCR using sequence-specific primers enables efficient genotyping of clinical samples

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Abstract
We have developed a polymerase chain reaction method using sequence‐specific primers (PCR‐SSP) for rapid and correct genotyping of the common Lewis (FUT3) gene mutations 59T>G, 202T>C, 314C>T, 508G>A, and 1067T>A. The PCR‐SSP method was validated on 20 healthy blood donors and 16 non‐insulin‐dependent diabetic patients. All individuals were in parallel genotyped by our established polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) analysis. The FUT3 genotypes, determined with the PCR‐SSP method, were in complete accordance with the results of the PCR‐RFLP reference method. The PCR‐SSP method could also be adapted to assign the presence of a specific mutation to the respective FUT3 alleles. We found the method to be reliable, rapid and cheap with no requirements for restriction enzyme processing. Hum Mutat 18:358–359, 2001.
Funding Information
  • Swedish Medical Research Council
  • SSF-GLIBS, the Medical Faculty of Göteborg University
  • Swedish Society for Medical Research
  • Göteborg Medical Society
  • Wilhelm and Martina Lundgren’s Research Foundation (8266)

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