A Study of the Aminoacyl-sRNA Synthetases of Phaseolus vulgaris in Relation to Germination
Open Access
- 1 January 1969
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 44 (1) , 60-68
- https://doi.org/10.1104/pp.44.1.60
Abstract
Optimum conditions for the extraction and assay by ATP-pyrophosphate exchange of the aminoacyl-sRNA synthetases of the various tissues of french bean (Phaseolus vulgaris) seeds and seedlings are described. Extracts of plumules, after passage through Sephadex G-25, were assayed for synthetase activity using an amino acid mixture as substrate, when a 30 to 100-fold stimulation of exchange above the endogenous level was obtained. This marked enhancement of exchange by added amino acids is largely attributed to the use of dilute extracts.Keywords
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