Ethylene Oxide Sterilization of Tissue Culture Media
- 27 December 1963
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 142 (3600) , 1654-1655
- https://doi.org/10.1126/science.142.3600.1654
Abstract
Liquid ethylene oxide, which kills bacteria, yeasts, and molds, was added to a tissue culture medium instead of the usual antibiotics. With some limitations, the use of ethylene oxide sterilization of tissue culture medium has been shown feasible. Data indicate that this method can be used in experiments involving the sterilization of test chemicals and components of the medium used for mammalian cells in culture.Keywords
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