Expression of feline immunodeficiency virusgag gene inEscherichia coli
- 1 September 1992
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 122 (3-4) , 383-390
- https://doi.org/10.1007/bf01317200
Abstract
Thegag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed inEscherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIVgag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.Keywords
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